| | City | Population |
| 1. | Asuncion | 1,482,200 |
| 2. | San Lorenzo | 227,876 |
| 3. | Capiata | 198,553 |
| 4. | Lambare | 126,377 |
| 5. | Fernando de la Mora | 120,167 |
| 6. | Limpio | 96,143 |
| 7. | Nemby | 94,641 |
| 8. | Encarnacion | 74,983 |
| 9. | Colonia Mariano Roque Alonso | 72,008 |
| 10. | Itaugua | 64,997 |
| 11. | Villa Elisa | 64,099 |
| 12. | San Antonio | 55,754 |
| 13. | Caaguazu | 54,808 |
| 14. | Presidente Franco | 54,292 |
| 15. | Coronel Oviedo | 51,286 |
| 16. | Concepcion | 48,123 |
| 17. | Villarrica | 41,157 |
| 18. | Caacupe | 21,696 |
| 19. | Ita | 18,459 |
| 20. | Ayolas | 12,061 |
| 21. | Santa Rita | 11,823 |
| 22. | Colonia General Alfredo Stroessner | 11,558 |
| 23. | Aregua | 11,222 |
| 24. | San Isidro de Curuguaty | 11,095 |
| 25. | Horqueta | 10,531 |
| 26. | Piribebuy | 10,337 |
| 27. | Paraguari | 10,079 |
| 28. | Tobati | 9,952 |
| 29. | Ypacarai | 9,655 |
| 30. | San Pedro de Ycuamandiyu | 9,360 |
| 31. | Capitan Bado | 9,114 |
| 32. | Guarambare | 9,065 |
| 33. | Eusebio Ayala | 8,253 |
| 34. | Filadelfia | 8,048 |
| 35. | San Juan Nepomuceno | 7,893 |
| 36. | Benjamin Aceval | 7,751 |
| 37. | Doctor Juan Leon Mallorquin | 7,481 |
| 38. | Salto del Guaira | 7,385 |
| 39. | Santa Rosa | 7,257 |
| 40. | Yaguaron | 7,181 |
| 41. | Repatriacion | 7,085 |
| 42. | Obligado | 6,350 |
| 43. | Emboscada | 5,911 |
| 44. | Carapegua | 5,784 |
| 45. | San Pedro del Parana | 5,722 |
| 46. | Bella Vista | 5,653 |
| 47. | Colonia Menno | 5,615 |
| 48. | Nanawa | 5,587 |
| 49. | Arquitecto Tomas Romero Pereira | 5,533 |
| 50. | Hohenau | 5,306 |
| 51. | Quiindy | 5,137 |
| 52. | Puerto Rosario | 5,123 |
| 53. | San Jose | 5,117 |
| 54. | San Alberto | 4,989 |
| 55. | Doctor Juan Manuel Frutos | 4,797 |
| 56. | Yuty | 4,774 |
| 57. | Atyra | 4,768 |
| 58. | Altos | 4,763 |
| 59. | Puerto Mayor Otano | 4,701 |
| 60. | San Bernardino | 4,651 |
| 61. | Ybycui | 4,629 |
| 62. | Iturbe | 4,475 |
| 63. | Las Palomas | 4,383 |
| 64. | Carmen del Parana | 4,196 |
| 65. | General Artigas | 4,073 |
| 66. | Colonia Catuete | 4,070 |
| 67. | Pirayu | 4,000 |
| 68. | Fram | 3,916 |
| 69. | Itacurubi del Rosario | 3,887 |
| 70. | Itacurubi de la Cordillera | 3,576 |
| 71. | Capiibary | 3,537 |
| 72. | Colonia Yguazu | 3,363 |
| 73. | Acahay | 3,205 |
| 74. | Itaquyry | 3,038 |
| 75. | Abai | 3,024 |
| 76. | General Elizardo Aquino | 2,983 |
| 77. | Nueva Italia | 2,841 |
| 78. | Trinidad | 2,837 |
| 79. | Colonia Neuland | 2,816 |
| 80. | Ygatimi | 2,809 |
| 81. | Los Cedrales | 2,754 |
| 82. | Antequera | 2,701 |
| 83. | La Colmena | 2,551 |
| 84. | Jesus | 2,530 |
| 85. | Capitan Miranda | 2,498 |
| 86. | Fuerte Olimpo | 2,475 |
| 87. | Carayao | 2,473 |
| 88. | Arroyos y Esteros | 2,455 |
| 89. | Tacuati | 2,405 |
| 90. | Colonia Mauricio Jose Troche | 2,368 |
| 91. | Bella Vista | 2,334 |
| 92. | Villa Florida | 2,305 |
| 93. | Mbocayaty | 2,296 |
| 94. | Caapucu | 2,284 |
| 95. | Natalio | 2,252 |
| 96. | Guayaybi | 2,247 |
| 97. | San Cosme y Damian | 2,223 |
| 98. | Mbuyapey | 2,201 |
| 99. | Naranjal | 2,151 |
| 100. | Santiago | 2,144 |
The Source for World Rankings