| | City | Population |
| 1. | Guatemala City | 994,938 |
| 2. | Mixco | 473,080 |
| 3. | Villa Nueva | 406,830 |
| 4. | Petapa | 141,455 |
| 5. | San Juan Sacatepequez | 136,886 |
| 6. | Quetzaltenango | 132,230 |
| 7. | Villa Canales | 122,194 |
| 8. | Escuintla | 103,165 |
| 9. | Chinautla | 97,172 |
| 10. | Chimaltenango | 82,370 |
| 11. | Chichicastenango | 79,759 |
| 12. | Huehuetenango | 79,426 |
| 13. | Amatitlan | 71,836 |
| 14. | Totonicapan | 69,734 |
| 15. | Santa Catarina Pinula | 67,994 |
| 16. | Santa Lucia Cotzumalguapa | 62,097 |
| 17. | Puerto Barrios | 56,605 |
| 18. | San Francisco El Alto | 54,493 |
| 19. | Coban | 53,375 |
| 20. | San Jose Pinula | 47,247 |
| 21. | San Pedro Ayampuc | 46,803 |
| 22. | Jalapa | 45,834 |
| 23. | Coatepeque | 45,654 |
| 24. | Solola | 45,373 |
| 25. | Mazatenango | 44,132 |
| 26. | Chiquimula | 41,521 |
| 27. | San Pedro Sacatepequez | 40,021 |
| 28. | Antigua Guatemala | 39,368 |
| 29. | Retalhuleu | 36,656 |
| 30. | Zacapa | 36,088 |
| 31. | Jutiapa | 34,332 |
| 32. | Jacaltenango | 34,084 |
| 33. | Santiago Atitlan | 33,309 |
| 34. | Momostenango | 31,739 |
| 35. | Palin | 31,329 |
| 36. | San Benito | 30,764 |
| 37. | Barberena | 30,539 |
| 38. | Ciudad Vieja | 30,203 |
| 39. | Ostuncalco | 28,894 |
| 40. | Fraijanes | 28,492 |
| 41. | Nahuala | 27,690 |
| 42. | Cantel | 26,063 |
| 43. | Panzos | 25,569 |
| 44. | San Marcos | 25,088 |
| 45. | Santiago Sacatepequez | 24,210 |
| 46. | La Gomera | 24,001 |
| 47. | Santa Cruz del Quiche | 23,618 |
| 48. | Nebaj | 23,301 |
| 49. | Tecpan Guatemala | 21,978 |
| 50. | Sumpango | 20,884 |
| 51. | Comalapa | 20,738 |
| 52. | Esquipulas | 20,674 |
| 53. | Flores | 20,464 |
| 54. | Chicacao | 20,426 |
| 55. | San Pablo Jocopilas | 20,259 |
| 56. | Comitancillo | 19,669 |
| 57. | San Cristobal Verapaz | 19,664 |
| 58. | Gualan | 19,354 |
| 59. | Nuevo San Carlos | 19,162 |
| 60. | Colomba | 19,115 |
| 61. | Morales | 18,994 |
| 62. | Patzun | 18,704 |
| 63. | Puerto San Jose | 18,655 |
| 64. | San Andres Itzapa | 18,647 |
| 65. | Palencia | 18,574 |
| 66. | Pueblo Nuevo Tiquisate | 18,189 |
| 67. | San Lucas Sacatepequez | 17,959 |
| 68. | Jocotenango | 17,918 |
| 69. | Alotenango | 17,410 |
| 70. | Poptun | 17,320 |
| 71. | Chisec | 17,018 |
| 72. | Patzicia | 16,494 |
| 73. | Cuilapa | 16,484 |
| 74. | La Esperanza | 16,461 |
| 75. | Sanarate | 15,843 |
| 76. | El Estor | 15,842 |
| 77. | El Tejar | 15,770 |
| 78. | Asuncion Mita | 15,608 |
| 79. | Santa Maria de Jesus | 15,529 |
| 80. | El Palmar | 15,167 |
| 81. | Malacatan | 14,923 |
| 82. | San Sebastian | 14,823 |
| 83. | Santa Catarina Ixtahuacan | 14,734 |
| 84. | San Lucas Toliman | 14,545 |
| 85. | Livingston | 14,350 |
| 86. | San Pedro Sacatepequez | 14,315 |
| 87. | Patulul | 14,196 |
| 88. | Barillas | 14,100 |
| 89. | San Francisco Zapotitlan | 13,855 |
| 90. | San Miguel Chicaj | 13,747 |
| 91. | San Pablo | 13,668 |
| 92. | Guastatoya | 13,467 |
| 93. | San Pedro Carcha | 13,332 |
| 94. | San Mateo Ixtatan | 13,213 |
| 95. | Joyabaj | 13,164 |
| 96. | Santa Lucia Milpas Altas | 13,001 |
| 97. | Santa Barbara | 12,906 |
| 98. | Panajachel | 12,863 |
| 99. | Chiquimulilla | 12,842 |
| 100. | San Andres Xecul | 12,133 |
The Source for World Rankings